BIOS 242 Week 3 Assignment; Lab 6 of 14 Onsite; Carbohydrate Fermentation

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Contributor

Matthew Lillard

Category

Biosciences

Course

BIOS 242 Fundamentals Of Microbiology With Lab

Institute

Chamberlain

Pages

4

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Lab 6: Carbohydrate Fermentation Learning Objectives: Define fermentation and develop a knowledge of fermentation end products. Understand the theory behind the carbohydrate fermentation procedure. Understand the various components of the carbohydrate media and how these components work. Appropriately analyze results for a variety of microorganisms. In the clinical laboratory, one technique used to identify bacteria is the carbohydrate fermentation tests. Bacteria have the ability to utilize different types of carbohydrates; if the bacteria possess the necessary enzymes to break down those carbohydrates; they will produce acidic end-products and in some cases CO2 gas. In this laboratory, we will determine the carbohydrate fermentation profiles of different bacteria. The media used in this laboratory contains Trypticase that will allow for the growth of all bacteria. Trypticase is a peptone derived from the breakdown of proteins and supports the growth of a variety of bacteria. Breakdown of protein results in the production of ammonia which increases the pH of the medium making it alkaline. The broth will contain 0.5% to 1% of a single desired carbohydrate (such as lactose, glucose, and sucrose). Finally, a pH indicator, phenol red is added to the broth to detect the

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